HPTLC and 1H-NMR as Fingerprints to Spot Ingredients of a Polyherbal Medicne Bhunimbadi Kvatha Churna
Introduction: Bhūnimbadi Kvātha Cūrṇa (BKC) is a polyherbal medicine formulated from eight ingredients viz. plant of Swertia chirata Buch. Ham., root of Aconitum heterophyllum Wall. ex. Royle, stem bark of Symplocos racemosa Roxb., rhizome of Cyperus rotundus Linn., seed of Holarrhena antidysenterica Wall., root of Coleus vettiveroides. K. C. Jacob, fruit of Coriandrum sativum Linn. and fruit pulp of Aegle marmelos Corr. It is used for the treatment of asthma, cough, fever due to pitta dosa, and bleeding disorders according to Ayurvedic Formulary of India. The current study is focused on identification of ingredients and their formulation BKC using qualitative high performance thin layer chromatography (HPTLC) and 1H-nuclear magnetic resonance (1H-NMR) spectra fingerprint. Methods: Diagnostic HPTLC method is optimised to spot each ingredient in BKC. 1H-NMR spectroscopy of total methanolic extract in DMSO was also carried out to detect the presence of corresponding signals from ingredients. Results: HPTLC as well as 1H-NMR was found to be effectual in identifying ingredients of BKC. The results can be employed to identify BKC of different manufacturers of herbal markets. Conclusion: The test can also be employed in pharmacopoeias for quality control of multi-herb formulations used in Ayurveda.